The celeb duo, who welcomed son Silas Randall back in April, got a baby sitter for their wee one and put on their best duds to attend the GLSEN Respect Awards on Oct. 23 in Los Angeles, Calif.
During the awards show, JT took to the stage and joked about a rare night without their son.
"Thank you for allowing mommy and daddy to have a really good night out together."
The superstar singer, who was joined onstage by his actress wife, added, "Honey, look around, this is what adults look like. They do exist! They don't smell like poopy diapers. …some of you do."
The two were at the event to receive the Inspiration Award, which they were presented with for their commitment to improving the lives of children, as well as their efforts to help the gay, lesbian, bisexual and transgender community.
During their acceptance speech, the new mom took a moment to be grateful to the education network for all its efforts.
"We want to thank you for leading the way to make our schools more safe, [and for] inspiring us for all of our children," the 33-year-old said.
For the glittering event, Biel opted for an on-trend gothic-inspired dress from Balmain's Spring/Summer '16 collection. Always one to put on a good show, Timberlake went for a glitzy tuxedo jacket.
After accepting their award, the couple, who wed back in 2012, stopped by Balmain creative director Olivier Rousteing's birthday bash at a private home in Los Angeles.
The soiree was attended by a cast of celebs, including Kim Kardashian, Kylie Jenner, Kendall Jenner, Kourtney Kardashian, Cara Delevingne, Jennifer Lopez, Jada Pinkett Smith, Willow Smith and Jaden Smith.