Don't mess with the Queen!

Prince William may be second in line to the throne, but he's not excluded from getting a good old fashioned scolding from Her Majesty.

The video — which is hilarious, by the way — shows William sitting down during last weekend's Trooping the Colour ceremony from the Buckingham Palace balcony. Queen Elizabeth II was having no part of her grandson sitting on his derrière as she and the rest of the royal family stood.

As William was kneeling down and laughing with his son, Prince George, the Queen was seen gently hitting Will on the arm and can clearly be seen mouthing the words, "Stand up, William" to him.

Prince William, who is surrounded by his wife, Duchess Kate, daughter Princess Charlotte, 1, and brother Prince Harry, immediately does as commanded and stands up. But, the best part of the video, arguably, is Prince George's adorable reaction, where he put his hand over his face.

Whether he did this because he's simply a 2-year-old boy and was shielding his eyes from a flyover or because he was in dismay over the scolding isn't known, but we'd prefer to think it was the latter.

Tony Clark / Splash News

Trooping the Colour is the annual tradition to celebrate Queen Elizabeth II's official birthday.

This isn't the first time that Will has been told off by his grandmother. In a documentary called "The Queen At 90," Will spoke of a childhood incident that has stuck with him. At the time, Will was riding a bike with his cousin Peter Phillips and they were chasing Peter's sister Zara around the Balmoral Castle in Scotland.

"We were chasing Zara around who was on a go-cart, and Peter and I managed to herd Zara into a lamppost and the lamppost came down and nearly squashed her," he said. "I remember my grandmother being the first person out at Balmoral running across the lawn in her kilt. She came charging over and gave us the most almighty b----ing, and that sort of stuck in my mind from that moment on."

She does, though, have a softer and humorous side (as evidenced by this epic photobombing).

"She's been a very strong female influence and having lost my mother at a young age, it's been particularly important to me that I've had somebody like the Queen to look up to, who's been there and who has understood some of the more, um, complex issues when you lose a loved one," Will said in the documentary. "So she's been incredibly supportive and I've really appreciated her guidance."

Still, you don't cross the boss.